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An alternative method for the genetic transformation of sweet orange

Identifieur interne : 003021 ( Main/Exploration ); précédent : 003020; suivant : 003022

An alternative method for the genetic transformation of sweet orange

Auteurs : G. H. Fleming [États-Unis] ; O. Olivares-Fuster [États-Unis] ; S. Fatta Del-Bosco [États-Unis] ; J. W. Grosser [États-Unis]

Source :

In vitro cellular & developmental biology. Plant [ 1054-5476 ] ; 2000.

RBID : Pascal:01-0164242

Descripteurs français

Pascal (Inist)
- Transformation génétique, Méthode, Protoplaste, Protéine fluorescente verte, Régénération, Efficacité traitement, Milieu culture, Citrus sinensis.

English descriptors

KwdEn :
- Citrus sinensis, Culture medium, Genetic transformation, Green fluorescent protein, Method, Protoplast, Regeneration, Treatment efficiency.

Abstract

An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of Itaborai' sweet orange isolated from an embryogenic nucellar-derived suspension culture. Following protoplast culture in liquid medium and transfer to solid medium, transformed calluses were identified via expression of the green fluorescent protein, physically separated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. Transgenic plantlets were recovered from germinating somatic embryos and by in vitro rooting of shoots. To expedite transgenic plant recovery, regenerated shoots were also micrografted onto sour orange seedling rootstocks. Presence of the transgene in calluses and regenerated sweet orange plants was verified by gene amplification and Southern analyses. Potential advantages of this transformation system over the commonly used Agrobacterium methods for citrus are discussed.

Affiliations:

Links toward previous steps (curation, corpus...)

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to stream PascalFrancis, to step Checkpoint: 000977
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to stream Main, to step Curation: 003021

Le document en format XML

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<front><div type="abstract" xml:lang="en">An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of Itaborai' sweet orange isolated from an embryogenic nucellar-derived suspension culture. Following protoplast culture in liquid medium and transfer to solid medium, transformed calluses were identified via expression of the green fluorescent protein, physically separated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. Transgenic plantlets were recovered from germinating somatic embryos and by in vitro rooting of shoots. To expedite transgenic plant recovery, regenerated shoots were also micrografted onto sour orange seedling rootstocks. Presence of the transgene in calluses and regenerated sweet orange plants was verified by gene amplification and Southern analyses. Potential advantages of this transformation system over the commonly used Agrobacterium methods for citrus are discussed.</div>
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An alternative method for the genetic transformation of sweet orange

An alternative method for the genetic transformation of sweet orange

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri